In 1976, Guilbert and Iscove tested that precursor cells of erythrocytes and macrophages may be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium.
Iscove's Medium is a version of Dulbecco's Modified Eagle's Medium (DMEM) containing: Selenium, additional amino acids and vitamins, Sodium pyruvate, HEPES buffer, Potassium nitrate instead of ferric nitrate.
Further studies demonstrated that Iscove's Medium would support: Murine B lymphocytes, Hematopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, a variety of hybrid cells.
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Iscove’s Modified DMEM w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes
Article-No : SPI-114-1L
Sterilty : Non Sterile
Volume : 1L, 10L, 50L
Storage : +2/+8 °C
State : Powder